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. 2019 Jan 25;9(3):900–919. doi: 10.7150/thno.29515

Figure 2.

Figure 2

CircHMGCS1 is remarkably upregulated in HB cells and tissues. (A) The genomic loci of circHMGCS1 in the HMGCS1 gene, which is located at chromosome 5p12. (B) Expression levels of cytoplasmic control transcripts (18S and GAPDH), the nuclear control transcript (U1), and circHMGCS1 were determined by qRT-PCR in the cytoplasmic and nuclear fractions of HepG2 cells. (C) Relative expression of circHMGCS1 in human normal hepatocyte cell lines (L-O2 and HL-7702), human HB cell lines (HepG2 and HUH6) and human hepatocellular carcinoma cell lines (Bel-7404 and SMMC-7721) as measured by qRT-PCR (one-way analysis of variance, Dunnett's test). (D) Relative expression of circHMGCS1 from HB and normal tissue pairs were measured by qRT-PCR (n = 37). N, normal tissues and T, tumor tissues (paired-samples t test). (E) Grading criteria based on the percentage of cells with different staining intensities at two magnifications (100× and 200×) used to define the circHMGCS1 expression level in tissue sections determined by ISH. (F) The Pearson's χ2 test of circHMGCS1 in 27 HB and paired normal tissue pairs. (G) Representative pictures of circHMGCS1 staining in tissue sections at three magnifications (100×, 200× and 400×). *p < 0.05, **p < 0.01, and ***p < 0.001.