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. 2019 Jan 25;9(3):900–919. doi: 10.7150/thno.29515

Figure 4.

Figure 4

CircHMGCS1 affects the proliferation and apoptosis abilities of HB cells. (A) CircHMGCS1 silencing efficiency using siRNAs in HepG2 and HUH6 cells was verified by qRT-PCR (one-way analysis of variance, Dunnett's test). (B-E) HepG2 and HUH6 cells transfected with si_circHMGCS1-1 or si_circHMGCS1-2 were subjected to CCK8 assays (one-way analysis of variance, Dunnett's test) (B and C) and colony formation assays (D and E) to evaluate the role of circHMGCS1 in cell proliferation and viability. (F-G) Apoptosis analysis of HUH6 cells transfected with si_NC, si_circHMGCS1-1 or si_circHMGCS1-2 using flow cytometry assays (one-way analysis of variance, Dunnett's test). (H) The expression of circHMGCS1 was detected by qRT-PCR in HepG2 and HUH6 cells infected with lentivirus expressing circHMGCS1 and mock vector (independent-samples t test). (I-J) The proliferation ability of HepG2 and HUH6 cells were measured by colony formation assays (independent-samples t test). (K-L) The growth curves of cells were measured by CCK8 assays (independent-samples t test). *p < 0.05, **p < 0.01, and ***p < 0.001.