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. 2018 Nov 2;132(21):2299–2322. doi: 10.1042/CS20180563

Figure 9. 3-MA treatment inhibits activation of Wnt-1 and Notch pathway in hyperuricemic rats.

Figure 9

Kidney tissue lysates collected after 3 weeks of daily feeding of the mixture of adenine and potassium oxonate with or without 3-MA administration were prepared and subjected to immunoblot analysis with antibodies to Wnt1, β-catenin, and GAPDH (A). Expression levels of Wnt1 was quantitated by densitometry and normalized with GAPDH (B). Expression levels of β-catenin were quantitated by densitometry and normalized with GAPDH (C). Kidney tissue lysates were prepared and subjected to immunoblot analysis with antibodies to Notch1, Jagged-1, and GAPDH (D). Expression levels of Notch1 was quantitated by densitometry and normalized with GAPDH (E). Expression levels of Jagged-1 were quantitated by densitometry and normalized with GAPDH (F). Data are represented as the mean ± S.E.M. Means with different superscript letters are significantly different from one another (P<0.05).