Table 1.
Antibodies used for immunohistochemistry
| Antibody | Product Code | Specificity | Genea | Host/Clone | Dilution | Source |
|---|---|---|---|---|---|---|
| Desmin | M0760 | Human and animal desmin | DES | mAb-mouse/ D33 | 1:100 | 1 |
| Desmin | 18–0016 | Human desmin | DES | mAb-mouse/ ZC18 | 1:1000 | 2 |
| Desmin | ab15200 | Human and animal desmin | DES | pAb-rabbit | 1:2000 | 3 |
| Dystrophin | GTX15277 | Human dystrophin (C-terminus) | DMD | pAb-rabbit | 1:7500 | 4 |
| Dystrophin | NCL-DYS1 | Human dystrophin (Rod domain) | DMD | mAb-mouse/ Dy4/6D3 | 1:5 | 5 |
| Dystrophin | NCL-DYS2 | Human dystrophin (C-terminus) | DMD | mAb-mouse/ Dy8/6C5 | 1:10 | 5 |
| Dystrophin | NCL-DYS3 | Human dystrophin (N terminus) | DMD | mAb-mouse/ DY10/12B2 | 1:10 | 5 |
| Alpha-actinin | A7732 | Human and animal α-actinin (Sarcomeric) | ACTN2 | mAb-mouse/ EA-53 | 1:500 | 6 |
| Laminin | PC 128 | Human laminin | LAM | pAb-sheep | 1:15000 | 7 |
| Utrophin | sc-33700 | Human utrophin | UTRN | mAb-mouse/ 8A4 | 1:200 | 8 |
| Slow MyHC | A4.840 | Human and animal MyHCI | MyH7 | mAb-mouse | 1:400 | 9 |
| Fast A MyHC | A4.74 | Human and animal MyHCIIa | MyH2 | mAb-mouse | 1:500 | 9 |
aOfficial gene nomenclature according to OMIM. (http://www.ncbi.nlm.nih.gov/omim). 1. Dako, Sweden, 2. Invitrogen Corporation, CA, USA; 3. Abcam, UK, 4. GeneTex Inc., Taiwan, 5. Novocastra Laboratories Ltd., UK, 6. Sigma-Aldrich Co, UK; 7. Binding site Inc., USA; 8. Santa Cruz Biotechnology Inc. UK, 9. Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by the University of Biological Sciences, Iowa City, Iowa, USA