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. 2019 Feb 14;20:53. doi: 10.1186/s12882-019-1239-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a-h Images from the first biopsy. a Endocapillary proliferative glomerulonephritis (H&E 400x). b Ultrastructural examination with electron dense immune-type deposits with lack of organized substructure (25,000x). c Direct immunofluorescence study shows bright granular IgG3. d Weak IgG1 e Negative IgG2. f Negative IgG4. g Bright kappa. h Negative lambda staining (all IF images 400x). i-m Images from second biopsy. i Mild mesangial expansion and hypercellularity (H&E 400x). j IgG staining shows absence of granular staining (400x). k Kappa trace staining. l Lambda negative (both 400x). m No electron dense deposits on ultrastructural examination (6000x). n-o Images from third biopsy. n Changes of transplant glomerulopathy (H&E 400x). o Ultrastructural examination with subendothelial widening, electron lucent amorphous material and entrapped non-specific electron densities (30,000x)