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. 2019 Feb 15;14:19. doi: 10.1186/s13000-019-0794-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Histologic analysis of laser-induced thermal therapy treated left temporal glioblastoma multiforme revealed distinct histologic zones. Hematoxylin and eosin stained sections represent neoplastic tissue surrounded by granulation tissue and necrosis. Section images were captured (original magnification 4X) and collaged. Higher-magnification of a necrotic area (Zone 1), b granulation tissue (Zone 2) and d tumor (Zone 3). c Macrophages detected by CD68 immunohistochemistry in granulation tissue. e Glial fibrillary acidic protein stained astrocytes (f, g and h). Single cells with positivity for f Ki67, g p53 and h Olig2 consistent with viable glioma