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. 2019 Feb 14;7:20. doi: 10.1186/s40478-019-0665-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Protein expression of SGmic and SGmac markers in freshly isolated microglia and spleen monocytes/macrophages. (a) Protein expression of SGmic and SGmac genes and reference markers (Cx3cr1, Cd11b, CD45, Ccr2) in microglia and spleen monocytes/macrophages. IBAQ intensities of each protein normalized to Gapdh intensity are shown. (b) Proteomic data were analyzed by a column-wise analysis using a two-sample t-test and a Benjamini-Hodgberg-based FDR < 0.05. T-test difference of SGmic, SGmac and reference marker expression in microglia relative to spleen monocytes/macrophages is shown (n = 4)