Table 1.
Activation of α7 by Amyloid β peptide (Aβ) in the presence of N-(5-Chloro-2,4-dimethoxyphenyl)-N′-(5-methyl-3-isoxazolyl)-urea (PNU)-120596.
| [ACh] (μM) | Aβ | [Aβ] (nM) | % active patches (n) | Mean cluster duration (ms) |
|---|---|---|---|---|
| 100 | No | 0 | 100 (6) | 2263 ± 990 |
| 0 | Aβ1–40 | 0.1 | 42.9 (7) | 762 ± 316 |
| 0 | 1 | 22.2 (18) | 2016 ± 1364 | |
| 0 | 10 | 30.0 (10) | 5229 ± 1392* | |
| 0 | 100 | 0 (9) | Nd | |
| 0 | Aβ1–42 | 0.1 | 77.8 (9) | 1401 ± 805 |
| 0 | 1 | 46.2 (13) | 1208 ± 946 | |
| 0 | 10 | 33.3 (9) | 1751 ± 766 | |
| 0 | 100 | 0 (6) | Nd |
Single-channel currents from cells expressing human α7 were recorded in the presence of 1 μM PNU-120596 plus 100 μM acetylcholine (ACh) or Aβ at the indicated concentrations. The mean cluster duration was obtained from the corresponding histograms. Nd: not detected. Statistical comparisons were performed against the corresponding control condition using analyses of variance (ANOVA; Dunnett’s multiple comparisons test). *p < 0.05.