Effects of RANKL and TRAIL on NF-κB activation in HASMCs. Smooth
muscle cells were treated for 72 h with RANKL (0–25 ng/ml), TRAIL
(0–25 ng/ml), or a combination of both ligands (see key below). Cells
were then monitored by Western blotting for activation of NF-κB as
follows: (A) p52/p100 ratio; and (B) Phosphop65/ p65
ratio. Histograms represent fold change in band intensity relative to untreated
controls. Blots are representative. *P ≤.05 versus 0
ng/ml RANKL (or control). Key: 5R, 5 ng/ml RANKL; 25R, 25 ng/ml RANKL; 5T, 5
ng/ml TRAIL.