Effects of RANKL and TRAIL on NF-κB activation
in a HAEC:HASMC transwell co-culture model. HAECs were treated for 72 h with
RANKL (0–25 ng/ml), TRAIL (0–25ng/ml), or a combination of both
ligands (see key below). HASMCs were then harvested and monitored by Western
blotting for activation of NF-κB as follows: (A)
p52/p100 ratio; and (B) Phospho-p65/p65 ratio. Histograms represent
fold change in band intensity relative to untreated controls. Blots are
representative. Key: 5R, 5 ng/ml RANKL; 25R, 25 ng/ml RANKL; 5T, 5 ng/ml
TRAIL.