Table 2.

SAXS analysis of LsALDH16 and HsALDH16A1

	LsALDH16			HsALDH16A1
	2.0 mg/ml	4.0 mg/ml	6.0 mg/ml	1.0 mg/ml	1.6 mg/ml	3.2 mg/ml
Guinier Analysis
Rg (Å)	35.6 ± 1.0	36.1 ± 0.4	35.0 ± 0.6	36.3 ± 1.0	37.5 ± 0.9	37.6 ± 0.5
qmin(Å−1)	0.015887	0.015330	0.018675	0.015887	0.011985	0.014215
qRg range	0.57 – 1.28	0.55 – 1.30	0.65 – 1.22	0.58 – 1.29	0.45 – 1.29	0.53 – 1.29
R-square	0.9972	0.9991	0.9984	0.9899	0.9930	0.9980
P(r) analysisa
Rg (Å)	35.5 ± 0.2	35.68 ± 0.06	35.22 ± 0.04	36.65 ± 0.08	37.50 ± 0.06	37.48 ± 0.06
Dmax (Å)	109	112	106	109	112	115
q-range (Å−1)	0.0159 – 0.3219	0.0153 – 0.3208	0.0187 – 0.3208	0.0159 – 0.3219	0.0131 – 0.3219	0.0148 – 0.3213
Total estimate	0.91	0.90	0.94	0.92	0.93	0.88
Porod Volume (Å3)	202000	204000	207000	230000	236000	237000
SAXS mass (kDa)b	141.5 (0.93)	151.3 (1.00)	149.2 (0.98)	147.1 (0.86)	156.0 (0.92)	170.1 (1.00)
χ2 from FoXSc	0.16	0.68	0.95	1.12	1.45	2.38
SASBDB code	SASDE47	SASDE57	SASDE67	SASDE87	SASDE97	SASDEA7

^aFrom calculations of the distance distribution function using GNOM via PRIMUS.

^bMolecular mass estimated from the SAXS MoW2 server [58]. The ratio of the experimental molecular mass to the expected value is given in in parentheses. For reference, the dimer masses of LsALDH16 and HsALDH16A1 are 151.6 kDa and 170.3 kDa, respectively.

^cGoodness-of-fit parameter from FoXS using a dimer model (Fig. 4).