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. 2019 Feb 11;13:601–609. doi: 10.2147/DDDT.S182087

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© 2019 Ma et al. This work is published and licensed by Dove Medical Press Limited

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Emodin induced the apoptosis of fibroblasts isolated from patients with AS.

Notes: Fibroblasts isolated from patients with AS were exposed to 10 µM emodin for 72 hours. (A) Apoptotic cells were detected with Annexin V and PI double staining. (B) The apoptotic cell rates were calculated (**P<0.01 compared with DMSO group). (C) Expressions of active caspase-3, active caspase-9, Bax, and Bcl-2 were analyzed by Western blotting in fibroblast cells. (D) Bax relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group). (E) Bcl-2 relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group). (F) Active caspase-9 relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group). (G) Active caspase-3 relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group).

Abbreviations: AS, ankylosing spondylitis; DMSO, dimethyl sulfoxide; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Emodin induced the apoptosis of fibroblasts isolated from patients with AS.

Notes: Fibroblasts isolated from patients with AS were exposed to 10 µM emodin for 72 hours. (A) Apoptotic cells were detected with Annexin V and PI double staining. (B) The apoptotic cell rates were calculated (**P<0.01 compared with DMSO group). (C) Expressions of active caspase-3, active caspase-9, Bax, and Bcl-2 were analyzed by Western blotting in fibroblast cells. (D) Bax relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group). (E) Bcl-2 relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group). (F) Active caspase-9 relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group). (G) Active caspase-3 relative expression was quantified by normalization of β-actin (**P<0.01 compared with DMSO group).

Abbreviations: AS, ankylosing spondylitis; DMSO, dimethyl sulfoxide; PI, propidium iodide; FITC, fluorescein isothiocyanate.