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. 2019 Feb 11;12:1119–1128. doi: 10.2147/OTT.S195529

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© 2019 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The expressions of MMP-2 and EMT key proteins were analyzed by Western blotting.

Notes: (A, B) The expression of MMP-2 in CM was examined upon treatment with rHsp90α (10 µg/mL), control IgG (10 µg/mL) or Hsp90α Ab (10 µg/mL) of ECA109 and ECA9706 for 24 hours by Western blotting. Coomassie blue staining was used as a loading control for CM. (C, D) ECA109 and ECA9706 were stimulated by the same treatment for 24 hours as that in (A) and (B). The expression of E-cadherin and N-cadherin in TCL was examined. Data shown as mean ± SD, *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: CM, conditioned media; Co.St, Coomassie blue staining; rHsp90α, recombinant Hsp90α protein; EMT, epithelial-to-mesenchymal transition; Hsp90α Ab, Hsp90α neutralizing antibody; TCL, total cell lysate.