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. Author manuscript; available in PMC: 2020 Feb 12.
Published in final edited form as: Chem Commun (Camb). 2019 Feb 12;55(14):2090–2093. doi: 10.1039/c8cc10319d

Fig. 1.

Fig. 1

Longer RNA selection on mineral surfaces. (A and B) Random 8-, 12-, 16-, 20-, and 24-mer RNAs (0.6 μM each) and 0.2 mg of each mineral were incubated at 22 °C for 2 h, and the concentration of each length RNA on the surfaces (A) and in each supernatant (B) was determined from radioactivity of 32P-labeled RNAs through gel electrophoresis. An example of an analyzed gel image is shown in Fig. S2 (ESI†). (C) RNA concentration on the surface for adsorption experiments performed with hydroxyapatite (HA) at 22 °C, 37 °C, and 48 °C. (D) An example of a gel image. Lanes 1, no mineral (NM), 22 °C; 2, +HA, 22 °C; 3, +HA, 37 °C; 4, +HA, 48 °C. In all panels, RNA concentrations were normalized to the levels of a control reaction (NM) performed at 22 °C; the error bars indicate standard errors (N = 3).