Table 1. The results of Hotelling’s T-squared test of Light-On VMR between TL strain at different stages.

Before integrated normalization
Comparison	Test statistic (p-value)
Stage (dpf)	Before light onset (-2–0 s)	After light onset (1–3 s)
3 vs. 6	115 (0.0000)	208 (0.000e+00)
3 vs. 9	5.83 (0.121)	535.7 (0.000e+00)
6 vs. 9	82.8 (0.0000)	13.6 (3.671e-3)
After integrated normalization
Comparison	Test statistic (p-value)
Stage (dpf)	Before light onset (-2–0 s)	After light onset (1–3 s)
3 vs. 6	13.2 (0.0132)	117.66 (0.000e+00)
3 vs. 9	0.188 (0.9795)	66.04 (8.071e-14)
6 vs. 9	11.4 (0.0152)	94.02 (0.000e+00)

The top table contains the test results before integrated normalization, whereas the bottom table contains the test results after integrated normalization. The corresponding activity plots can be found in Fig 3a and 3e respectively. In both tables, we presented the comparisons of VMR three seconds before light onset and three seconds after light onset.