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. Author manuscript; available in PMC: 2020 Jan 9.
Published in final edited form as: Cell Host Microbe. 2018 Dec 20;25(1):113–127.e6. doi: 10.1016/j.chom.2018.11.009

Figure 2. Increased abundance and translocation of lactobacilli in lupus.

Figure 2.

Fecal pellets from WT C57B1/6, TLR7.1 Tg, and C57B1/6 mice treated with IMQ were collected; bacterial DNA was isolated and sequenced. Linear Discriminant Analysis Effect Size (LDA) scores of altered taxa in (A) TLR7.1 Tg compared to WT mice and (B) C57B1/6 mice treated with or without IMQ. Shown are significantly altered taxa (increased in red, decreased in green). (C) Total percentage of bacterial translocation as determined by anaerobic and aerobic non-selective cultures. (D) Profile of translocating bacterial species. (E) Distribution of Lactobacillus spp. translocation in MLN, liver, and spleen from WT, TLR7.1 Tg, and TLR7.1 Tg mice treated with or without ABX. (F) Relative abundances of fecal L. reuteri in TLR7.1 Tg compared to WT mice and (G) C57B1/6 mice treated with or without IMQ evaluated by 16S rDNA gene sequencing. (H) Relative abundances of fecal Lactobacillus spp. in normal healthy donors (NHD) and systemic lupus erythematosus (SLE) patients. The results are expressed as mean ± SEM (n=3–20 mice and 28–50 human samples per group). Data are representative of at least three independent experiments. *P<0.05 was considered statistically significant; **P<0.01; ***P<0.001; ****P<0.0001; ns=not significant; W0=week of disease onset. See also Figure S2 and Tables S1 and S2.