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. Author manuscript; available in PMC: 2020 Jan 9.
Published in final edited form as: Cell Host Microbe. 2018 Dec 20;25(1):113–127.e6. doi: 10.1016/j.chom.2018.11.009

Figure 4. L. reuteri exacerbates lupus-related pathogenesis.

Figure 4.

(A-B) SPF C57B1/6 mice were treated with IMQ and were gavaged with De Man, Rogosa and Sharpe broth (MRS), L. reuteri (LR), or L. johnsonii (LJ) primary tissue isolates harvested from TLR7.1 Tg mice. Mice were sacrificed for organ weights and immunologic analyses. (A) Weights of spleen and liver. (B) Relative mRNA expression of type I IFN in spleen and ileum. (C-D) TLR7.1 Tg mice were gavaged with MRS or LR. Mice were sacrificed for organ weights and immunologic analyses. (C) Weights of spleen and liver. Cells from spleen and PP were isolated and analyzed by flow cytometry. (D) Frequencies of pDCs in spleen and PP. (E-I) GF C57B1/6 mice were treated with IMQ and monocolonized with LR. Mice were sacrificed for organ weights, immunologic, and histologic analyses. (E) Weights of spleen and liver. (F) Relative mRNA expression of type I IFN in spleen and ileum. Cells from spleen, MLN, and SI-LP were isolated and analyzed by flow cytometry. (G) Frequencies of pDCs in spleen, MLN, and SI-LP. (H) Representative H&E staining of kidney tissue. (I) Histopathologic scoring for glomerulonephritis. Q) Quantification of proteinuria. The results are expressed as mean ± SEM (n=3–16 mice per group). The results are representative of at least two independent experiments. *P<0.05 was considered statistically significant; **P<0.01; ***P<0.001; ****p<0.0001; ns=not significant. See also Figure S4.