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. 2019 Feb 15;14(2):e0212337. doi: 10.1371/journal.pone.0212337

Fig 5. Exogenous α-actinin-2 and Huntingtin associate in lysates from human HeLa cells and deletion analysis maps the interaction region to aa 399–969 of Huntingtin.

Fig 5

(a) Deletion analysis was performed in HeLa cells using Huntingtin cDNA constructs as depicted co-expressed with a cDNA for GFP-α-actinin-2 (GFP-ACTN2). The CAG length in Huntingtin was 18 (wild-type). Ability to pull down GFP-α-actinin-2 indicated at right. (b) Western blots of FLAG immunoprecipitates and inputs. FLAG tagged full length Huntingtin (FLAG-HTT-1-3144) or aa 1–969 (FLAG-HTT-1-969) pulls down GFP-α-actinin-2. (c) FLAG tagged full length Huntingtin (FLAG-HTT-1-3144) or aa 399–1518 (FLAG-HTT-399-1518) pulls down GFP-α-actinin-2. The minimal shared region was aa 399–969 of Huntingtin. Full blots for b and c are shown in S5 Fig.