Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Drosophila melanogaster) | UAS-LT3-Dam | A Brand | NA | UAS drives mCherry from 1st cistron and Dam from 2nd cistron |
| Strain, strain background (D. melanogaster) | engrailed-Gal4 | A Brand | NA | Expressed in row 6 and 7 neuroblasts |
| Strain, strain background (D. melanogaster) | R19B03AD;R18F07DBD | G Rubin | NA | Expression in NB7-4 lineage from stage 9 |
| Strain, strain background (D. melanogaster) | Lbe(K)-Gal4 | S Thor | NA | Expression in NB5-6 lineage from stage 9; salivary gland at stage 17 |
| Strain, strain background (D. melanogaster) | UAS-LT3-Dam:Hb | This paper | NA | UAS drives mCherry from 1st cistron and Dam:Hb from 2nd cistron |
| Strain, strain background (D. melanogaster) | Sca-Gal4 | Y Hiromi | NA | Expressed in all NBs |
| Strain, strain background (D. melanogaster) | hsFLP;;UAS-MCFO | A Nern | NA | MCFO (multi-colored-flip-out) line |
| Strain, strain background (D. melanogaster) | MARCM stock | T Lee | NA | For clonal analysis of NB7-4 lineage |
| Strain, strain background (D. melanogaster) | UAS-HA:UPRT | Doe lab | NA | Control transgene |
| Strain, strain background (D. melanogaster) |
Da-Gal4 | BDSC | 55850 homozygous on III | |
| Antibody | chicken anti-GFP (polyclonal) |
Abcam (Eugene, OR) | ab13970 | (1:1000) |
| Antibody | mouse anti-en (monoclonal) |
DSHB (Iowa City, IA) 4D9 | (1:50) | |
| Antibody | rabbit anti-Dan (polyclonal) |
Doe lab | NA | (1:1000) |
| Antibody | rabbit anti-Hb (polyclonal) |
Doe lab | NA | (1:400) |
| Antibody | rabbit anti-Eve (polyclonal) |
Doe lab | SC1320A | (1:500) |
| Antibody | rat anti-Gsb (monoclonal) |
Holmgren Lab | 1:1 10E10/16F2 | (1:10) |
| Antibody | mouse anti-mCherry (polyclonal) |
Clonetech | 632543 | (1:500) |
| Antibody | rabbit anti-V5::549 (polyclonal) |
Rockland | 600-442-378 | (1:400) |
| Antibody | mouse anti-HA::488 (monoclonal) |
Cell signaling | 2350S | (1:200) |
| Antibody | rat anti-Ollas::650 (monoclonal) |
Novus | NBP1-06713 | (1:200) |
| Antibody | Secondary antibodies (polyclonal) |
Thermofisher (Eugene, OR) |
(1:400) |