Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) |
E. coli MG1655 | E. coli genetic stock center |
CGSC #6300 | |
| Sequence-based reagent |
tRNASer northern probe |
IDT | GATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTC | |
| Sequence-based reagent |
tRNAGly northern probe |
IDT | GAATCGAACCCGCATCATCAGCTTGG | |
| Sequence-based reagent |
tRNALeu northern probe |
IDT | GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTG | |
| Sequence-based reagent |
NEB Universal miRNA Cloning Linker |
New England BioLabs |
Cat# S1315S | 5′ rAppCTGTAGGCACCATCAAT–NH2 3′ |
| Sequence-based reagent |
RT Primer | IDT | /5Phos/AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC/iSP18/CACTCA/iSp18/TTCAGACGTGTGCTCTTCCGATCTATTGATGGTGCCTACAG | |
| Sequence-based reagent |
PCR Primer Forward |
IDT | AATGATACGGCGACCACCGAGATCTACAC | |
| Sequence-based reagent |
PCR Primer Reverse |
IDT | CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCG | |
| Peptide, recombinant protein |
Nuclease S7 (MNase) |
Millipore Sigma | Cat# 10107921001 | |
| Peptide, recombinant protein |
T4 polynucleotide kinase |
New England BioLabs |
Cat# M0201S | |
| Peptide, recombinant protein |
T4 RNA Ligase 2, truncated |
New England BioLabs |
Cat# M0242S | |
| Peptide, recombinant protein |
SuperScript III |
ThermoFisher Scientific |
Cat# 18080085 | |
| Peptide, recombinant protein |
CircLigase ssDNA Ligase |
Lucigen | Cat# CL4115K | |
| Commercial assay or kit |
Ribo-Zero rRNA Removal Kit (Bacteria) |
Illumina | Cat# MRZB12424 | |
| Chemical compound, drug |
Mupirocin | Millipore Sigma | Cat# M7694 | |
| Chemical compound, drug |
Chloramphenicol | Millipore Sigma | Cat# C0378 | |
| Chemical compound, drug |
Transfer Ribonucleic Acid: Lysine specific |
Chemical Block Ltd | NA | |
| Other | MOPS EZ Rich Defined Medium Kit |
Teknova | Cat# M2105 | (Growth Media) |