Figure 7. Proteome analyses reveal additional molecular markers for circalunar phase with distinct expression profiles and proposed functions.

(a–a’’’) The neuropeptide Whitnin, a’- peristomeal cirrus, a’’- head, a’’’- indicted head expression in a’’ with different focus. (b–b’’) The oxygen storage protein Haemerythrin, b’’- head, b’- lateral head aspects below the focal plane of b’’. (c–c’’) The iron storage protein Ferritin. c’- peristomeal cirrus (a–c) compares the normalised protein expression profiles (top, ‘proteomics’) with the corresponding DESeq2 normalised mRNA expression profile (bottom, ‘RNA-Seq’). All graphs show the arithmetic mean with standard deviation, and the individual data points. (a’–c’’) Expression domains as characterised by whole-mount in situ hybridisation; (a’’,b’’,c’’) dorsal views of immature Platynereis heads stained with riboprobes of the corresponding transcript; All images are oriented with the anterior side to the top; scale bar: 250 µm. a.e.: anterior eye, p.e.: posterior eye.

Figure 7—figure supplement 1. Platynereis Whitnin is part of a Proctolin/Whitnin family of neuropeptides.

Maximum likelihood phylogeny of Proctolin/Whitnin family, see Figure 7—source datas 1 and 2 for alignment and Accession numbers.