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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Cell Rep. 2014 Dec 18;9(6):2027–2033. doi: 10.1016/j.celrep.2014.11.037

Figure 3). The CBF3 complex and the Dam1-DASH complex are required for Cse4 localization at LacO.

Figure 3)

A) Model of the natural kinetochore. B-I) The quantification of ChIP-qPCR averaged from at least three experiments ± S.D. * represents statistical significance (t test, p< .05). B) The ChIP of Ndc10-GFP with anti-GFP antibody. C) The ChIP of GFP-Cse4 with anti-GFP antibody, comparing asynchronous cells with kinetochore mutations at 37°C. D) The ChIP of GFP-Cse4 with anti-GFP antibody, comparing asynchronous to metaphase-enriched cells. E-I) The ChIP of GFP-Cse4 with anti-GFP antibody, comparing asynchronous cells with kinetochore mutations at 37°C. J) Model of the synthetic kinetochore.