Table 2.
Electrophysiology parameters of the six haplotypes and I962V (IV) and L1092P (LP) variants
| Current density | Voltage dependent activation | Voltage dependent inactivation | Late current | Time course of recovery from inactivation | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pA/pF | V1/2 (mV) | k (mV) | n | V1/2 (mV) | k (mV) | n | INa, L (%) | INa,L (pA/pF) | n | τ, f (ms) | τ, s (ms) | n | |
| PT | −24.6 ± 5.1 | 11.8 ± 1.8* | 12.5 ± 1.1 | 7 | −68.5 ± 1.3 | 10.4 ± 1.1 | 6 | 6.4 ± 1.2* | −3.3 ± 0.8 | 5 | 17.3 ± 2.2 | 348.6 ± 79.3* | 5 |
| VA+LP | −60.1 ± 12.5 | −2.3 ± 1.3* | 8.0 ± 1.1 | 7 | −70.1 ± 2.6 | 10.4 ± 2.3 | 6 | 12.0 ± 1.8 | −7.2 ± 1.5 | 5 | 10.3 ± 2.7 | 226.5 ± 75.1 | 3 |
| WT | −25.6 ± 6.7 | 3.0 ± 1.0 | 10.5 ± 0.8 | 7 | −68.9 ± 1.4 | 12.2 ± 1.3 | 6 | 11.7 ± 1.6 | −5.5 ± 1.0 | 7 | 10.7 ± 2.7 | 124.5 ± 34.7 | 6 |
| VA | −53.4 ± 8.7* | −6.3 ± 1.4* | 8.5 ± 1.3 | 6 | −70.5 ± 1.1 | 9.3 ± 1.0 | 5 | 13.5 ± 3.2 | −7.8 ± 1.8 | 6 | 20.2 ± 5.8 | 326.5 ± 115.6 | 6 |
| IV+VA+LP | −44.2 ± 12.1 | −3.5 ± 1.0* | 10.2 ± 0.9 | 6 | −74.0 ± 3.6 | 13.4 ± 2.7 | 6 | 20.2 ± 3.3* | −8.7 ± 1.8+ | 5 | 11.7 ± 4.5 | 245.0 ± 100.0 | 4 |
| IV+VA | −26.1 ± 6.7 | 1.1 ± 1.1 | 10.2 ± 0.9 | 8 | −67.6 ± 2.3 | 10.9 ± 2.2 | 6 | 22.4 ± 0.8* | −9.7 ± 2.2+ | 5 | 7.5 ± 1.6 | 167.9 ± 36.2 | 5 |
| IV | −33.6 ± 11.3 | 2.8 ± 1.1 | 10.1 ± 0.9 | 7 | −70.2 ± 1.7 | 10.1 ± 1.5 | 6 | 15.4 ± 1.7 | −6.3 ± 2.4 | 3 | 4.2 ± 1.6 | 84.6 ± 16.3 | 3 |
| LP | −17.8 ± 3.5 | 8.3 ± 2.1 | 12.4 ± 1.4 | 7 | −70.7 ± 2.4 | 12.1 ± 2.1 | 4 | 12.9 ± 3.1 | −5.3 ± 1.4 | 6 | 11.5 ± 2.4 | 196.2 ± 53.8 | 6 |
Current density at +10 mV, INaL (%) is percentage of INaL and INaL (pA/pF) at +20 mV. τf and τs are the fast and slow time constants for recovery from inactivation, respectively. Data displayed as mean ± standard error, n=number of cells.
*:
statistically different from WT (P>0.05).
+:
significant difference compared to PT haplotype (P<0.05).