. Author manuscript; available in PMC: 2019 Oct 16.

Published in final edited form as: Immunity. 2018 Oct 9;49(4):627–639.e6. doi: 10.1016/j.immuni.2018.08.019

Table 1 –

Colony-forming activity of CD51⁻ TNCs

Colony assay	Cell population	Colony-forming activity per 10³ cells ± SEM	n
CFU-C	CD44⁺ CD51⁻ TNCs	0.0 ± 0.0	5
	CD44⁻ CD51⁻ TNCs	0.0 ± 0.0	5
	LSK	35.6 ± 13.9	5

BFU-E	CD44⁺ CD51⁻ TNCs	0.0 ± 0.0	6
	CD44⁻ CD51⁻ TNCs	0.0 ± 0.0	6
	CFU-E	0.0 ± 0.0	5
	preCFU-E	2.0 ± 1.4	5
	preMegE	9.8 ± 2.9	5

CFU-E	CD44⁺ CD51⁻ TNCs	0.6 ± 0.4	5
	CD44⁻ CD51⁻ TNCs	0.0 ± 0.0	5
	CFU-E	13.4 ± 1.2	5
	preCFU-E	4.2 ± 3.1	5
	preMegE	0.0 ± 0.0	5

CFU pre-B	CD44⁺ CD51⁻ TNCs	6.2 ± 1.9	6
	CD44⁻ CD51⁻ TNCs	0.0 ± 0.0	6
	CD11b⁻ Gr-1⁻ CD3ε⁻	1.1 ± 0.1	6
	Ter119⁻ B220⁺	1.1 ± 0.1	6

Cell populations were sorted (500-2,000 cells) from C57BL/6 mice bone marrow and plated in methylcellulose (Stem Cell Technologies, Cat#: 3434 (CFU-C), Cat#: 3436 (BFU-E), Cat#: M3334 (CFU-E) Cat#: 3630 (CFU pre-B ⁺ 25ng/mL SCF)) and incubated for 7 days (BFU-E, CFU pre-B) or 2-3 days (CFU-E) at 37°C in 5% CO ₂. CFU-C, colony-forming unit-cell; BFU-E, burst-forming unit-erythroid; CFU-E, colonyforming unit-erythroid; TNCs: CD45⁻ Ter119⁻ CD31⁻ cells; CFU-E cell population: Lin⁻ Sca-1⁻ c-Kit⁺ CD41⁻ CD16/32⁻ CD105⁺ CD150⁻; pre-CFU-E cell population: Lin⁻ Sca-1⁻ c-Kit⁺ CD41⁻ CD16/32⁻ CD105⁺ CD150⁺; preMegE: Lin⁻ Sca-1⁻ c-Kit⁺ CD41⁻ CD16/32⁻ CD105⁻ CD150⁺). LSK: Lineage⁻ (B220⁻, CD11b⁻, Gr-1⁻, CD3ε⁻ and Ter119⁻) Sca-1⁺ c-Kit⁺.