(A) Representative immunoblots show CHX chase assays using T98G cells stably expressing LIN52-V5 or LIN52-S28A-V5 proteins alone, or together with HA-B-Myb. (B, C) Quantitative analysis of LIN52-V5 and LIN52-S28A-V5 CHX chase assays shown in 3A. In panel B, graph represents average ± stdev (N=5) of LIN52 LIN52-S28A band density in control cells without B-Myb overexpression. LIN52 band density was first normalized to actin and then plotted relative to 0h (** - p<0.01). In panel C, graph shows the average change in LIN52 band density at 3h compared to 0h, in the presence of HA-B-Myb relative to that in the control cells. Note that LIN52-V5 stability in the presence of HA-B-Myb is significantly greater than in cells expressing LIN52-V5 alone (N=3, * - p<0.05) whereas LIN52-S28A-V5 is not significantly affected by HA-B-Myb.