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. Author manuscript; available in PMC: 2019 Mar 11.
Published in final edited form as: Oncogene. 2018 Sep 11;38(7):1080–1092. doi: 10.1038/s41388-018-0490-y

Figure 4. Depletion of B-Myb in T98G cells increases turnover of LIN52 but not the S28A-LIN52 mutant.

Figure 4.

(A) Representative immunoblots show that RNAi knockdown of B-Myb decreases stability of wild type LIN52, but not LIN52-S28A-V5. T98G cells stably expressing LIN52 proteins were transfected with siNT (non-targeting) or B-Myb-specific siRNA, and used for CHX chase assays after 36 hours. Note the relative stability of LIN9 and LIN37 compared with that of LIN52. Asterisk indicates non-specific band. (B) Graph shows average change in LIN52 band density at 3h compared to 0h, in siB-Myb transfected cells relative to that in siNT cells. Note that LIN52-V5 stability in the presence of siB-Myb is significantly lower than in siNT-transfected cells (N=3, * - p<0.05) whereas LIN52-S28A-V5 is not significantly affected.