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. Author manuscript; available in PMC: 2019 Mar 18.
Published in final edited form as: Oncogene. 2018 Sep 18;38(7):1121–1135. doi: 10.1038/s41388-018-0498-3

Figure 1. Loss of INPP4B changes AR transcriptional activity.

Figure 1.

(A) Three dimensional rendering of INPP4B distribution. Cells were fixed and stained for INPP4B (white) and counterstained with DAPI (blue). (B) LNCaP cells were transfected with control (Ctrl) or two independent INPP4B specific siRNAs (INPP4B-1, INPP4B-2) for 48 hours in medium supplemented with FBS. Protein levels of AR, INPP4B, and tubulin were compared by Western blotting. (C) LNCaP cells were transfected as in (B). RNA was extracted and used in microarray analysis. Changes in gene expression were compared to AR regulated genes in LNCaP cells. (D) GSEA of INPP4B regulated genes using an AR transcriptional signature in LNCaP cells (GSE60721). (E-K). LNCaP cells were transfected with either control or INPP4B siRNAs for 48 hour intervals. Forty eight hours later cells were placed in medium supplemented with 10% CSS and treated with either vehicle (ethanol) or 0.1 nM R1881 for 24 hours as indicated. RNA was analyzed for expression of INPP4B (E), KIAA1324 (F), SYTL2 (G), TARP (H), TMPRSS2 (I), PSA (J), and NNMT (K). Expression was normalized to 18S. ** p < 0.01, *** p < 0.001.