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. Author manuscript; available in PMC: 2019 Mar 18.
Published in final edited form as: Oncogene. 2018 Sep 18;38(7):1121–1135. doi: 10.1038/s41388-018-0498-3

Figure 2. Reciprocal regulation of AR-V7 and INPP4B.

Figure 2.

(A) LNCaPAR-V7/pHage cells were placed in medium with 10% CSS or 10% TET FBS serum for 24 hours and treated with 50 ng/ml Dox or 1 nM R1881 as indicated for an additional 36 hours. Protein level of INPP4B, AR-FL, AR-V7, pAkt, pPKCβII, and tubulin were assessed by Western blotting. (B, C) LNCaPAR-V7/pHage cells were transfected with control or INPP4B siRNA and treated with either vehicle or 25 ng/ml Dox for additional 24 hours. Gene expression of INPP4B (B) and EDN2 (C) were analyzed by RT-qPCR using 18S as a control. (D-I) LNCaPAR-V7/pHage cells were transfected and treated as in B. RNA was analyzed for expression of NNMT (D), PSA (E), TARP (F), TMPRSS2 (G), KIAA1324 (H), and SYTL2 (I).*** p < 0.001.