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. Author manuscript; available in PMC: 2019 Mar 18.
Published in final edited form as: Oncogene. 2018 Sep 18;38(7):1121–1135. doi: 10.1038/s41388-018-0498-3

Figure 3. INPP4B transcription is regulated by full length AR and FOXA1.

Figure 3.

LNCaP cells in 10% CSS were transfected with noncoding control or FOXA1 specific siRNA; cells were treated with R1881 after 24 hours. Cells were harvested after 48 hours and assayed for FOXA1 expression by western blotting (A), RNA isolated and assayed for FKBP5 (B), RASSF3 (C), and INPP4B (D). (E) A diagram of AR, FOXA1, and Pol2 recruitment and RNA-seq in LNCaP cells. (F-J) LNCaPAR-V7/pHage cells were placed in medium with 10% CSS for 24 hours and treated with 50 ng/ml Dox or 10 nM R1881 overnight as indicated. Recruitment of AR-FL and AR-V7 to PSA enhancer (F), INPP4B intron 2 (G), INPP4B enhancer region (H), INPP4B transcription start site chr4:143522349 (I), and chr4:143514774 (J) were measured by ChIP-qPCR using Igg as control. * p < 0.05, ** p < 0.01, *** p < 0.001.