Skip to main content
. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Pain. 2019 Mar;160(3):619–631. doi: 10.1097/j.pain.0000000000001442

Figure 5:

Figure 5:

ELISA quantification of ET-1 and β-endorphin in conditioned medium from 4T1-luc2 cells, SK-MEL-28 cells as well as 4T1-luc2 cells transduced with Ednrb (adEdnrb) or GFP (adGFP). A) 4T1-luc2 cells released increasing levels of ET-1 over 48 hours. After 48 h, conditioned medium from 4T1-luc2 cells contained significantly more ET-1 compared with conditioned medium from the non-painful melanoma cell line SK-MEL-28. B) Conditioned medium from the 4T1-luc2 cell line transduced with Ednrb released significantly more β-endorphin, compared with non-transduced and GFP-transduced controls. P < 0.05. Results analyzed by One-Way ANOVA followed by Bonferroni’s post hoc comparisons. n=2–3.