Figure 6:

Fewer DRG neurons respond to supernatant from 4T1-luc2 cells with re-expressed Ednrb. A) Representative Ca²⁺ transients from DRG neurons before and after the application of supernatant from 4T1-luc2 cells transduced with adEdnrb B) Re-expression of Ednrb results in a significant decrease in percent of DRG neurons that respond to supernatant compared with adGFP and non-transduced conditioned medium. C) Examples of Ca²⁺ traces from DRG neurons before and after the application of supernatant from 4T1-luc2 cells transduced with adGFP. A Ca²⁺change ≥ 30% of baseline was considered a response to treatment. n=3. ** P < 0.01. Results analyzed by One-Way ANOVA, followed by Bonferroni post hoc comparisons.