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Naloxone reversed the adEdnrb-induced suppression of DRG response to cancer supernatant. A) Examples of Ca2+ traces in DRG applied cancer supernatant from 4T1-luc2 cells transduced with adEdnrb neurons before (pre) and after (post) the application of 10 μM naloxone B) Inhibition of opioid signaling with 10uM naloxone increased the percentage of Ca2+ responsive DRG neurons. n=3. ** P < 0.01. Results analyzed by Two-way ANOVA followed by Bonferroni’s post hoc comparisons.
