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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Pain. 2019 Mar;160(3):702–711. doi: 10.1097/j.pain.0000000000001449

Figure 1. C5a produces dose-dependent mechanical hypersensitivity.

Figure 1.

(A) Paw withdrawal thresholds in WT (C57BL/6J) mice, as measured before (time=0 hr) and after injection of 10 ng (green circles; n=4), 100 ng (red squares; n=4), or 500 ng (black triangle; n=5) of C5a (in 10 µL of saline) into the plantar surface of the hindpaw. Significant main effect of time: F5,50=18.31; p<0.001 (two-way repeated-measures ANOVA). *p<0.05, **p<0.01, ***p<0.001, relative to baseline (Dunnett post hoc test). Color of each asterisk denotes significance of change relative to baseline for the corresponding test group. (B-D) Dose response curves were calculated based on the experiments in (A) using non-linear, four parameter regression model for 500 ng (B), 100 ng (C), and 10 ng (D) C5a injection. Horizontal dotted black line indicates 50% withdrawal frequency.