(A and B) Pre-treatment with the NK1 receptor antagonist SR140333 via subcutaneous administration (SC; 1 nmol; n=7) 30 min prior to C5a injection (500 ng) did not significantly affect C5a-induced mechanical (A) or thermal (B) hypersensitivity relative to vehicle-treated mice (n=6) (two-way repeated-measures ANOVA with Holm-Sidak post hoc test). Significant main effect of time: F5,55=24.8, p<0.0001 for mechanical sensitivity and F1,11=95.12, p<0.0001 for thermal sensitivity. (C and D), Pre-treatment with the NK1 receptor antagonist, SR140333 via intraperitoneal administration (IP; 1 mg/kg; n=6) 30 min prior to C5a injection (500 ng) did not significantly affect C5a-induced mechanical (C) or thermal (D) hypersensitivity relative to vehicle-treated mice (n=5) (two-way repeated-measures ANOVA with Holm-Sidak post hoc test). Significant main effect of time: F4,32=43.73, p<0.0001 for mechanical sensitivity and F1,9=110, p<0.0001 for thermal sensitivity.