Table 1: The numbers of deuterons exchanged after 5 min deuteration in the His243-containing peptide under low and high osmolality conditions.
Mutation site(s) are underlined. Values are reported as mean ± standard deviation from at least two independent experiments.
| EnvZc construct | Reporter peptide | Deuterons exchanged in the His243-containing peptide (5 min deuteration) | |
|---|---|---|---|
| Low osmolality | High osmolality | ||
| Wild type* | MAGVSHDLRTPLTRIRL | 5.4 ± 0.1 | 4.3 ± 0.1 |
| H243A* | ADLRTPLTRIRL | 5.3 ± 0.1 | 5.2 ± 0.1 |
| A239T | MTGVSHDLRTPLTRIRL | 4.9 ± 0.0 | 3.9 ± 0.1 |
| T247R | MAGVSHDLRRPLTRIRL | 3.2 ± 0.2 | 2.7 ± 0.0 |
| A239T/T247R | TGVSHDLRRPLTRIRL | 4.3 ± 0.2 | 3.3 ± 0.1 |
| D244A | AGVSHALRTPLTRIRL | 3.5 ± 0.2 | 2.5 ± 0.2 |
*
Data were reproduced from Wang et al. (2012) with permission.
For EnvZc H243A mutant, two peptic peptides were identified (238–245 and 243–254). The deuterium uptake value reported here is for the peptide spanning residues 243–254.