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. 2018 Dec 7;97(2):909–921. doi: 10.1093/jas/sky459

Figure 5.

Figure 5.

The effects of oral infusion of sodium butyrate at 0.36 g/kg body weight once daily for 39 d on messenger RNA (mRNA) expression of genes involved in ruminal epithelial VFA absorption (a) and metabolism (b) in preweaning lambs. Rumen tissues were collected 2 to 3 h after the final SB infusion at the ages of 49 d in lambs. Quantitative RT-PCR results are expressed as relative mRNA expression (fold of Con group, normalized to GAPDH), and the data were analyzed by the 2−ΔΔCT method. The values are means ± SEM, n = 7. MCT1 = monocarboxylate transporter isoform 1; MCT4 = monocarboxylate transporter isoform 4; PAT1 = putative anion transporter isoform 1; DRA = downregulated in adenoma; NHE1 = sodium/proton exchanger isoform 1; NHE2 = sodium/proton exchanger isoform 2; NHE3 = sodium/proton exchanger isoform 3; HMGCS1 = 3-hydroxy-3-methylglutaryl-CoA synthase isoform 1; HMGCS2 = 3-hydroxy-3-methylglutaryl-CoA synthase isoform 2; HMGCL = 3-hydroxy-3-methylglutaryl-CoA lyase; BDH1 = β-hydroxybutyrate dehydrogenase-1; BDH2 = β-hydroxybutyrate dehydrogenase-2.