Fig. 4.

Real-time imaging of vRNP release from a Rab7-positive endosome. (A) IAV-QD625 (red) colocalization with a Rab7-ECFP–positive endosome (cyan) was tracked. (Scale bar: 5 µm.) (B) Sequential snapshots are shown for the separation of the QD signal and Rab7 signal in the rectangular region of A. Filled arrowheads indicate the colocalization of QD and Rab7 signals, arrows indicate virus-negative endosomes, and hollow arrowheads indicate the released vRNP. (C) Trajectories of the two fluorescent dots from B. (D) Fluorescence image of an infected cell treated with NH₄Cl. (Scale bar: 5 µm.) (E) Sequential images of the rectangular regions of D. (F) Trajectories of the QD625 and ECFP fluorescent signals in NH₄Cl-treated cells. Images were taken at 60× magnification objective lens under a confocal microscope.