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. 2019 Jan 25;116(7):2603–2611. doi: 10.1073/pnas.1818313116

Fig. 5.

Fig. 5.

Reduced proliferation and high SA-β-gal activity of p16INK4a-activated peritoneal macrophages. (A) Bioluminescence imaging of p16LUC/+ mice following intraperitoneal injection with empty (control, CNTL) or quiescent human NDF-embedded alginate beads. Representative image was acquired 21 d after bead injection. (B) Representative FACS analysis of peritoneal macrophages (Mac-1+F4/80+) from p16+/+ (WT) and p16tdTom/+ mice at day 21 after NDF-bead injection. (C) mRNA expression of p16INK4a by qRT-PCR in FACS-sorted tdTom− and tdTom+ peritoneal macrophages. Fold-difference was calculated with respect to the mRNA levels in the tdTom− fraction. (D) Quantification of EdU+ cells by immunofluorescence staining. (E) Representative image of SA-β-gal staining. (F) Quantification of SA-β-gal level in E. Throughout, error bars represent SEM. The statistical significance of differences was assessed using unpaired in C and paired two-tailed Student’s t tests (*P < 0.05, **P < 0.01) in D and F.