Fig. 3.

miR482- and miR2118b-mediated silencing is relieved in STTM482 and STTM2118b lines, respectively. (A) Bar plot of total 21-nt siRNA counts in NLRs in WT and STTM lines. Colored fractions correspond to sNLs. Statistically significant differences were found using the one-way ANOVA test followed by Tukey’s honestly significant difference (HSD) test at 95% confidence limits. (B) Abundance of 21-nt siRNAs along two sNLs (LRR1 and LRR2, two well-studied CNL genes targeted by miR482) and TAS5 loci. Positions corresponding to the cleavage sites of their miRNA triggers (miR482s and miR2118b, respectively) are indicated by dashed lines. Gray boxes indicate the position of exons. sRNA reads are normalized to the whole library with nRPMs and presented as the mean from three biological replicates.