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. 2019 Jan 28;116(7):2761–2766. doi: 10.1073/pnas.1807621116

Fig. 2.

Fig. 2.

Spatial-temporal expression of two NRPD1-siRNA target genes and AGO4:GFP. (A) Expression patterns of two NRPD1-siRNA target genes in the ECec group based on the LCM data reported by Belmonte et al. (14), showing AGL91 expression in the CZE and AGL40 expression in the CZE and peripheral endosperm. CZSC, chalazal seed coat; EP, embryo proper; MCE, micropylar endosperm; PEN, peripheral endosperm; SC, distal seed coat; SUS, suspensor. With each region (except the suspensor), five developmental stages (from left to right) are preglobular, globular, heart, linear cotyledon, and mature green. (B) Confocal images of seeds (at 6 DAP) exhibiting AGO4:GFP expression patterns in EP, SC, PEN, CZE, and CSC. GFP is shown in green; chlorophyll autofluorescence, in red. (Scale bars: 0.1 mm.)