Fig. 2.

Characterization of γδT cells in the oral mucosa. (A) FACS plots show γ-chain usage by gingival γδT cells from adult TcrdH2BeGFP mice. Bar graphs depict mean frequencies + SEM of γ-chain types of gingival γδT cells. Data are pooled from two to three independent experiments (n = 3–7 mice per experiment). (B) Expression of selected typical surface markers on γδT cells in the gingiva and skin epithelium. Gray filled histograms represent isotype controls (Ctl). Data representative of three independent experiments (n = 5 mice in each experiment). (C and D) Isolated gingival lymphocytes were stimulated ex vivo with phorbol myristate acetate (PMA) and ionomycin. Representative FACS plots of three independent experiments (n = 3–4 mice per experiment). Bar graphs present the mean frequencies + SEM of pooled data from six experiments. FACS plots and bar graphs show γδT cells expressing IL-17 or IFN-γ and the relative contribution of γδT cells to IL-17–expressing total leukocytes in the tissue. (E and F) IL-17 production analyzed directly ex vivo in gingival γδT cells from Il17-eGFP reporter mice. (E) IL-17⁺ cells among CD44^hi γδT cells. Bar graph depicts means + SEM of pooled frequencies from two independent experiments (n = 4 mice per experiment). (F) Frequencies of γδT cells and αβT cells among all IL-17⁺ cells in the gingival tissue, two independent experiments (n = 4 mice per experiment). Bar graph shows mean frequency + SEM of pooled data from two independent experiments.