Fig. 6.

Blocking ferroptosis protects the heart against I/R injury. (A and B) Cardiac nonheme iron levels (A) and cardiac Ptgs2 mRNA levels (B) were measured in mice subjected to sham surgery or 30-min cardiac ischemia followed by 24 h of reperfusion (I/R) (n = 6–8 mice per group). (C) Representative images (Left) and quantitative data (Right) for infarct size (IF) and relative area at risk (AAR) in heart sections obtained from mice subjected to 30 min/24 h I/R injury and treated with saline (control), Fer-1, or DXZ. (D–G) Serum LDH levels (D), AST levels (E), CK-MB levels (F), and cardiac Anp, Bnp, and Myh7 mRNA (G) measured in mice subjected to sham surgery or 30 min/24 h I/R injury and treated with saline, Fer-1, or DXZ (n = 6–8 mice per group). (H) Representative images of Masson’s trichrome staining of heart sections obtained from mice subjected to sham surgery or 30 min/4 wk I/R injury and treated with saline, Fer-1, or DXZ. (I) Cardiac mt-Cytb and mt-Atp6 mRNA levels were measured in mice subjected to sham surgery or 30 min/24 h I/R injury and treated with saline, Fer-1, or DXZ (n = 6–8 mice per group). Summary data are presented as the mean ± SEM. Significance in A and B was calculated using the Student’s t test; *P < 0.05; ***P < 0.001. Significance in C–G and I was calculated using a one-way ANOVA with Tukey’s post hoc test; groups labeled with different letters differed significantly (P < 0.05).