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. 2019 Jan 28;116(7):2701–2706. doi: 10.1073/pnas.1817885116

Fig. 4.

Fig. 4.

The glutathione cycle can rescue restrictions of glutamine-derived glutamate. (A) Scheme of glutamate-glutamine cycling and blockade of system A glutamine transporters by MeAIB. Details appear in SI Appendix, Fig. S4. (B) Representative traces of mEPSC recordings in primary cortical neurons treated with 25 μM BSO (24 h) and/or 25 mM MeAIB (2 h). (C and D) Distribution of mEPSC frequency and amplitude in cortical neurons treated with BSO and/or MeAIB. (E and F) Cumulative probability plots of mEPSC frequency and amplitude. MeAIB, which restricts glutamine-derived glutamate, inhibits mEPSC frequency (blue). Decreases in mEPSC frequency induced by MeAIB were rescued by pretreatment with BSO (green), which increased mEPSC frequency (red). BSO also improved the decrease in mEPSC amplitudes by MeAIB. (‡P < 0.0001 by Steel–Dwass all pairs test.)