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. 2018 Nov 8;22(2):143–156. doi: 10.1093/ijnp/pyy092

Figure 1.

Figure 1.

Chemical structure of n-isopropyl-3-(cyclopropylmethoxy)-4-difluoromethoxy benzamide (FCPR03) and experimental procedures. (A) Chemical structure of FCPR03 and inhibition (half maximal inhibitory concentration, nM) of FCPR03 on PDE4CAT (the core catalytic domains of human phosphodiesterase 4 [PDE4]). (B) After 7 days of acclimatization, mice were subjected to chronic unpredictable mild stress (CUMS) for 56 days. FCPR03, rolipram, or vehicle were administered (i.p.) once a day for 14 days (from week 7 to week 8). Sucrose preference test (SPT) was performed on day 56, other behavioral tests were carried out on day 57, and the mice were subsequently killed. Enzyme-linked immunosorbent assay (ELISA) assay, biochemical assays, and Golgi staining were performed after behavioral tests. MW, molecular weight; OFT, open field test; TST, tail suspension test; FST, forced swimming test.