Figure 2.

Antidepressant-like effect of n-isopropyl-3-(cyclopropylmethoxy)-4-difluoromethoxy benzamide (FCPR03) in chronic unpredictable mild stress (CUMS)-exposed mice. Mice were exposed to CUMS for 56 days, and the hippocampus extracts were then homogenized. Western blots were used to assess phosphodiesterase 4 (PDE)4B protein levels (A); the corresponding quantification data are shown in (B). Data are expressed as mean±SEM (n=5). ^##P<.01 vs vehicle group. After 2 weeks of treatment with FCPR03 (0.5 mg/kg or 1.0 mg/kg) or rolipram (1.0 mg/kg), sucrose preference ratio in the sucrose preference test (SPT) (C) and the immobility times in the forced swim test (FST) (D) and tail suspension test (TST) (E) were measured. Data are expressed as mean±SEM (n=8). ^##P<.01, ^###P<.001 vs vehicle group, ^*P<.05, ^**P<.01 vs CUMS group.