Figure 7.

n-Isopropyl-3-(cyclopropylmethoxy)-4-difluoromethoxy benzamide (FCPR03) enhanced the level of cyclic adenosine monophosphate (cAMP) and the phophorylated cAMP-response element-binding protein (p-CREB) in the hippocampus in chronic unpredictable mild stress (CUMS)-exposed mice. After 2 weeks of treatment with FCPR03 (0.5 or 1.0 mg/kg) or rolipram (1.0 mg/kg), the hippocampal tissues were homogenized, and an enzyme-linked immunosorbent assay (ELISA) kit was used to assess cAMP levels (A), while western blot was used to assess phosphorylated cAMP-response element-binding protein (CREB), total CREB, and brain-derived neurotrophic factor (BDNF) (B) levels in the hippocampus. The corresponding quantification data are shown in (C) and (D). Data are expressed as mean±SEM (n=5). ^##P<.01, ^###P<.001 vs vehicle group, ^*P<.05, ^**P<.01 vs CUMS group.