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. 2019 Feb 15;10(3):152. doi: 10.1038/s41419-019-1403-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The predicted miR-451a seed region in the upstream and the ORF of BAP31 gene. b When upstream 177 was blocked, the luciferase activity was significantly reduced by miR-451a (**P < 0.01). c The location of the predicted miR-451a seed region in BAP31 gene. The base changes and deletions in four mutations of BAP31 upstream 177 sequence. d The renilla luciferase relative activity in wild-type vector BAP31 upstream 177 site and mutations (Wt, wild-type; Mut1, mutation 1; Mut2, mutation 2; Mut3, mutation 3; Mut4, mutation 4). ORF, open reading frame