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. 2019 Feb 15;10(3):152. doi: 10.1038/s41419-019-1403-x

Fig. 4. Over-expressing MiR-451a or silencing BAP31 suppressed the proliferation of CRC cells and increased the total apoptosis of CRC cells.

Fig. 4

a BAP31 protein relative expression was decreased in HCT116 and SW620 cells on silencing BAP31 (**P < 0.01; NC, untreated; P-S, transferred psilencer-Scr; P-B, transferred psilencer-BAP31). b, c Over-expressing miR-451a or silencing BAP31 exhibited significant inhibitory effects on the proliferation of HCT116 (b) and SW620 (c) cells compared to the control and untreated cells (NC). d, e Over-expressing miR-451a or silencing BAP31 induced significant apoptosis in HCT116 and SW620 cells compared to the control cells. The apoptotic cells were stained brightly blue by Hoechst staining (d; scale bar, 50 μm) and green fluorescence by TUNEL staining (e; scale bar, 25 μm). (f) As detected by flow cytometry, total apoptosis was increased by 12.69 and 13.81% when over-expressing miR-451a or silencing BAP31, respectively, in HCT116 cells. The total apoptosis increased by 10.88 and 13.55%, respectively, when over-expressing miR-451a or silencing BAP31 in SW620 cells. CRC, colorectal cancer