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. 2019 Feb 15;10(3):152. doi: 10.1038/s41419-019-1403-x

Fig. 6. Over-expressing miR-451a or silencing BAP31 increased intracellular calcium concentration.

Fig. 6

a Cytosolic calcium-specific fluorescent dye Fluo-4AM was added into the cell culture medium to a final concentration of 10 μM to detect calcium ions in the cytoplasm 48 h after transfection with pcDNA-miR-451a, pcDNA-NC, psilencer-BAP31, and psilencer-Scr in HCT116 and SW620 cells (scale bar, 50 μm). b Cytosolic calcium concentration was 1.67- and 2.01-fold higher in HCT116 cells over-expressing miR-451a or silencing BAP31, respectively. c Cytoplasmic calcium concentration was 2.08- and 2.27-fold higher in SW620 cells over-expressing miR-451a or silencing BAP31, respectively ( × 200)