Fig. 4.

TM2.6 mutations in TRAAK and TREK1 increase single-channel open probability. a, b Channel opening of TRAAK, TRAAK G133D, TREK1, and TREK1 G171D at −80 mV. Channel activity was determined by NPo analyzed at −80 and +80 mV. c, d Single-channel openings of TRAAK, TRAAK G133D, TREK1 and TREK1 G171D at two membrane potentials. Currents were recorded in cell-attached patches held at pipette potentials of +80 and −80 mV. Pipette and bath solutions contained 150 mM KCl. e, f Mean open time for TRAAK, TRAAK G133D, TREK1, and TREK1 G171D. Average unitary conductance for TRAAK, TRAAK G133D, TREK1, and TREK1 G171D. g, h Current–voltage relationships of single channels. Mean ± standard deviation. Source data are provided as a Source Data file. Each data point represents one recording; center lines, medians; open circles, means; box limits, 25th and 75th percentiles; whiskers, standard deviation. Mann–Whitney, n = 6, *p < 0.05